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| 02.04.2021 | 18:00 | Bohemians Praha 1905 | - | FK Jablonec | 0:0 (0:0) | |
| 03.04.2021 | 14:00 | Sigma Olomouc | - | FK Pardubice | 0:1 (0:0) | |
| 16:00 | SFC Opava | - | 1. FC Slovácko | 1:2 (0:0) | ||
| 16:00 | Slovan Liberec | - | Baník Ostrava | 0:0 (0:0) | ||
| 18:30 | Sparta Praag | - | FK Teplice | 7:2 (4:1) | ||
| 04.04.2021 | 14:00 | FC Fastav Zlín | - | MFK Karviná | 1:2 (1:1) | |
| 14:00 | FK Mladá Boleslav | - | 1. FK Příbram | 0:0 (0:0) | ||
| 16:00 | Viktoria Plzeň | - | Dynamo České Budějovice | 2:1 (1:1) | ||
| 18:30 | Zbrojovka Brno | - | Slavia Praag | 0:0 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praag | 25 | 20 | 5 | 0 | 70:15 | 55 | 65 |
| 2 |
|
Sparta Praag | 25 | 17 | 3 | 5 | 57:28 | 29 | 54 |
| 3 |
|
FK Jablonec | 25 | 15 | 4 | 6 | 41:27 | 14 | 49 |
| 4 |
|
1. FC Slovácko | 25 | 14 | 5 | 6 | 46:26 | 20 | 47 |
| 5 |
|
Slovan Liberec | 25 | 12 | 7 | 6 | 37:22 | 15 | 43 |
| 6 |
|
Viktoria Plzeň | 25 | 13 | 4 | 8 | 44:30 | 14 | 43 |
| 7 |
|
FK Pardubice | 25 | 11 | 5 | 9 | 27:32 | -5 | 38 |
| 8 |
|
Baník Ostrava | 25 | 9 | 7 | 9 | 33:25 | 8 | 34 |
| 9 |
|
Sigma Olomouc | 25 | 8 | 10 | 7 | 30:26 | 4 | 34 |
| 10 |
|
Dynamo České Budějovice | 25 | 7 | 10 | 8 | 27:33 | -6 | 31 |
| 11 |
|
Bohemians Praha 1905 | 25 | 7 | 9 | 9 | 26:28 | -2 | 30 |
| 12 |
|
FC Fastav Zlín | 25 | 8 | 5 | 12 | 25:33 | -8 | 29 |
| 13 |
|
MFK Karviná | 25 | 7 | 8 | 10 | 27:38 | -11 | 29 |
| 14 |
|
FK Teplice | 25 | 6 | 5 | 14 | 27:53 | -26 | 23 |
| 15 |
|
FK Mladá Boleslav | 25 | 4 | 9 | 12 | 29:42 | -13 | 21 |
| 16 |
|
Zbrojovka Brno | 25 | 3 | 8 | 14 | 19:39 | -20 | 17 |
| 17 |
|
SFC Opava | 25 | 3 | 6 | 16 | 16:49 | -33 | 15 |
| 18 |
|
1. FK Příbram | 25 | 2 | 8 | 15 | 17:52 | -35 | 14 |
| Champions League Kwali. | |
| Europa League Kwali. | |
| Degradatie |
