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| 19.02.2009 | 20:00 | Fortuna Sittard | - | FC Den Bosch | 1:0 (0:0) | |
| 20.02.2009 | 20:00 | AGOVV | - | BV Veendam | 4:1 (1:0) | |
| 20:00 | FC Eindhoven | - | MVV Maastricht | 1:1 (0:1) | ||
| 20:00 | Go Ahead Eagles | - | SBV Excelsior | 1:1 (0:0) | ||
| 20:00 | SC Cambuur | - | FC Omniworld | 2:0 (0:0) | ||
| 20:00 | FC Dordrecht | - | Helmond Sport | 1:0 (1:0) | ||
| 20:00 | HFC Haarlem | - | RKC Waalwijk | 0:2 (0:0) | ||
| 20:00 | TOP Oss | - | VVV-Venlo | 4:2 (3:1) | ||
| 20:00 | FC Emmen | - | FC Zwolle | 1:0 (0:0) | ||
| 20:00 | RBC Roosendaal | - | Telstar | 1:0 (1:0) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
VVV-Venlo | 27 | 18 | 3 | 6 | 65:31 | 34 | 57 |
| 2 |
|
RKC Waalwijk | 27 | 12 | 14 | 1 | 47:24 | 23 | 50 |
| 3 |
|
SC Cambuur | 27 | 14 | 5 | 8 | 50:39 | 11 | 47 |
| 4 |
|
SBV Excelsior | 27 | 11 | 11 | 5 | 41:25 | 16 | 44 |
| 5 |
|
MVV Maastricht | 27 | 10 | 10 | 7 | 40:30 | 10 | 40 |
| 6 |
|
FC Zwolle | 27 | 11 | 7 | 9 | 42:40 | 2 | 40 |
| 7 |
|
Go Ahead Eagles | 27 | 10 | 10 | 7 | 26:29 | -3 | 40 |
| 8 |
|
HFC Haarlem | 27 | 12 | 4 | 11 | 33:38 | -5 | 40 |
| 9 |
|
Helmond Sport | 27 | 10 | 9 | 8 | 40:36 | 4 | 39 |
| 10 |
|
FC Den Bosch | 27 | 10 | 8 | 9 | 45:34 | 11 | 38 |
| 11 |
|
TOP Oss | 27 | 10 | 5 | 12 | 35:43 | -8 | 35 |
| 12 |
|
RBC Roosendaal | 27 | 9 | 7 | 11 | 32:33 | -1 | 34 |
| 13 |
|
AGOVV | 27 | 10 | 4 | 13 | 43:52 | -9 | 34 |
| 14 |
|
Fortuna Sittard | 27 | 8 | 7 | 12 | 29:41 | -12 | 31 |
| 15 |
|
Telstar | 27 | 7 | 9 | 11 | 34:42 | -8 | 30 |
| 16 |
|
FC Dordrecht | 27 | 7 | 8 | 12 | 30:32 | -2 | 29 |
| 17 |
|
FC Eindhoven | 27 | 6 | 12 | 9 | 44:54 | -10 | 27 |
| 18 |
|
FC Emmen | 27 | 7 | 6 | 14 | 33:53 | -20 | 27 |
| 19 |
|
BV Veendam | 27 | 7 | 8 | 12 | 32:46 | -14 | 26 |
| 20 |
|
FC Omniworld | 27 | 5 | 5 | 17 | 27:46 | -19 | 20 |
| Promotie | |
| Play-offs | |
| Play-offs | |
| Play-offs | |
| Play-offs |
