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| 23.04.2021 | 14:30 | SK Líšeň | - | Viktoria Žižkov | 1:1 (0:0) | |
| 24.04.2021 | 16:00 | FK Ústí nad Labem | - | FC Hradec Králové | 0:3 (0:1) | |
| 17:00 | FK Blansko | - | Vysočina Jihlava | 1:3 (1:1) | ||
| 17:00 | FK Dukla Praha | - | FC Sellier & Bellot Vlašim | 1:1 (1:0) | ||
| 17:00 | FK Fotbal Třinec | - | Slavoj Vyšehrad | 3:0 (1:0) | ||
| 17:00 | 1. SK Prostějov | - | MFK Chrudim | 1:1 (1:0) | ||
| 25.04.2021 | 17:00 | FK Varnsdorf | - | FK MAS Táborsko | 0:0 (0:0) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 20 | 14 | 4 | 2 | 42:16 | 26 | 46 |
| 2 |
|
SK Líšeň | 20 | 10 | 8 | 2 | 34:19 | 15 | 38 |
| 3 |
|
1. SK Prostějov | 20 | 10 | 6 | 4 | 33:26 | 7 | 36 |
| 4 |
|
FK Ústí nad Labem | 20 | 9 | 5 | 6 | 19:24 | -5 | 32 |
| 5 |
|
MFK Chrudim | 20 | 9 | 4 | 7 | 28:20 | 8 | 31 |
| 6 |
|
FC Sellier & Bellot Vlašim | 20 | 8 | 5 | 7 | 28:23 | 5 | 29 |
| 7 |
|
FK Dukla Praha | 20 | 7 | 7 | 6 | 29:23 | 6 | 28 |
| 8 |
|
Viktoria Žižkov | 20 | 8 | 3 | 9 | 27:30 | -3 | 27 |
| 9 |
|
Vysočina Jihlava | 20 | 7 | 4 | 9 | 30:33 | -3 | 25 |
| 10 |
|
FK Varnsdorf | 20 | 5 | 9 | 6 | 16:22 | -6 | 24 |
| 11 |
|
FK Fotbal Třinec | 20 | 6 | 5 | 9 | 25:28 | -3 | 23 |
| 12 |
|
FK Blansko | 20 | 5 | 5 | 10 | 22:26 | -4 | 20 |
| 13 |
|
FK MAS Táborsko | 20 | 5 | 5 | 10 | 17:22 | -5 | 20 |
| 14 |
|
Slavoj Vyšehrad | 20 | 1 | 2 | 17 | 9:47 | -38 | 5 |
| Promotie | |
| Degradatie |
