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| 08.05.2021 | 15:45 | FC Hradec Králové | - | FK Dukla Praha | 2:1 (2:1) | |
| 16:00 | FK Ústí nad Labem | - | FK Blansko | 2:1 (0:1) | ||
| 17:00 | MFK Chrudim | - | FK Fotbal Třinec | 0:2 (0:1) | ||
| 17:00 | 1. SK Prostějov | - | SK Líšeň | 1:3 (1:1) | ||
| 17:00 | Slavoj Vyšehrad | - | FK Varnsdorf | 1:2 (1:0) | ||
| 09.05.2021 | 10:15 | Viktoria Žižkov | - | FC Sellier & Bellot Vlašim | 3:1 (2:0) | |
| 17:00 | FK MAS Táborsko | - | Vysočina Jihlava | 2:2 (2:1) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Hradec Králové | 23 | 17 | 4 | 2 | 47:18 | 29 | 55 |
| 2 |
|
SK Líšeň | 23 | 12 | 9 | 2 | 40:22 | 18 | 45 |
| 3 |
|
1. SK Prostějov | 23 | 11 | 6 | 6 | 37:32 | 5 | 39 |
| 4 |
|
Viktoria Žižkov | 23 | 11 | 3 | 9 | 33:32 | 1 | 36 |
| 5 |
|
FK Ústí nad Labem | 23 | 10 | 5 | 8 | 22:28 | -6 | 35 |
| 6 |
|
FC Sellier & Bellot Vlašim | 23 | 9 | 6 | 8 | 35:30 | 5 | 33 |
| 7 |
|
FK Dukla Praha | 23 | 8 | 7 | 8 | 32:27 | 5 | 31 |
| 8 |
|
MFK Chrudim | 23 | 9 | 4 | 10 | 29:27 | 2 | 31 |
| 9 |
|
FK Fotbal Třinec | 23 | 8 | 5 | 10 | 28:30 | -2 | 29 |
| 10 |
|
Vysočina Jihlava | 23 | 7 | 7 | 9 | 36:39 | -3 | 28 |
| 11 |
|
FK Varnsdorf | 23 | 6 | 10 | 7 | 19:25 | -6 | 28 |
| 12 |
|
FK MAS Táborsko | 23 | 6 | 7 | 10 | 22:25 | -3 | 25 |
| 13 |
|
FK Blansko | 23 | 6 | 5 | 12 | 27:30 | -3 | 23 |
| 14 |
|
Slavoj Vyšehrad | 23 | 1 | 2 | 20 | 12:54 | -42 | 5 |
| Promotie | |
| Degradatie |
