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| 18.03.2022 | 17:00 | Zbrojovka Brno | - | SK Líšeň | 1:3 (0:2) | |
| 19.03.2022 | 10:15 | MFK Chrudim | - | SFC Opava | 0:0 (0:0) | |
| 10:15 | FK Fotbal Třinec | - | 1. SK Prostějov | 4:0 (0:0) | ||
| 10:15 | FK Dukla Praha | - | FK MAS Táborsko | 0:0 (0:0) | ||
| 10:15 | MFK Vyškov | - | FK Varnsdorf | 2:0 (1:0) | ||
| 18:00 | FK Ústí nad Labem | - | Viktoria Žižkov | 0:0 (0:0) | ||
| 20.03.2022 | 10:30 | Sparta Praag B | - | 1. FK Příbram | 1:1 (0:0) | |
| 15:00 | FC Sellier & Bellot Vlašim | - | Vysočina Jihlava | 3:1 (3:1) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Zbrojovka Brno | 19 | 14 | 2 | 3 | 40:19 | 21 | 44 |
| 2 |
|
FC Sellier & Bellot Vlašim | 19 | 11 | 2 | 6 | 41:26 | 15 | 35 |
| 3 |
|
SK Líšeň | 19 | 10 | 5 | 4 | 33:21 | 12 | 35 |
| 4 |
|
Sparta Praag B | 19 | 10 | 2 | 7 | 33:24 | 9 | 32 |
| 5 |
|
FK Varnsdorf | 19 | 9 | 4 | 6 | 34:25 | 9 | 31 |
| 6 |
|
SFC Opava | 19 | 8 | 5 | 6 | 28:21 | 7 | 29 |
| 7 |
|
FK Dukla Praha | 19 | 7 | 5 | 7 | 23:24 | -1 | 26 |
| 8 |
|
Vysočina Jihlava | 19 | 7 | 5 | 7 | 18:24 | -6 | 26 |
| 9 |
|
1. SK Prostějov | 19 | 8 | 2 | 9 | 18:30 | -12 | 26 |
| 10 |
|
MFK Vyškov | 19 | 7 | 3 | 9 | 33:29 | 4 | 24 |
| 11 |
|
FK MAS Táborsko | 19 | 6 | 6 | 7 | 20:22 | -2 | 24 |
| 12 |
|
FK Ústí nad Labem | 19 | 5 | 8 | 6 | 20:24 | -4 | 23 |
| 13 |
|
1. FK Příbram | 19 | 5 | 6 | 8 | 24:34 | -10 | 21 |
| 14 |
|
FK Fotbal Třinec | 19 | 6 | 2 | 11 | 20:34 | -14 | 20 |
| 15 |
|
MFK Chrudim | 19 | 3 | 6 | 10 | 19:31 | -12 | 15 |
| 16 |
|
Viktoria Žižkov | 19 | 2 | 5 | 12 | 15:31 | -16 | 11 |
| Promotie | |
| Play-offs | |
| Degradatie |
