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| 26.04.2022 | 17:00 | FK Varnsdorf | - | SK Líšeň | 0:1 (0:0) | |
| 27.04.2022 | 17:00 | FK Ústí nad Labem | - | FK Dukla Praha | 1:1 (0:0) | |
| 17:00 | 1. FK Příbram | - | MFK Chrudim | 0:0 (0:0) | ||
| 17:00 | 1. SK Prostějov | - | FK MAS Táborsko | 1:2 (1:1) | ||
| 17:00 | MFK Vyškov | - | FC Sellier & Bellot Vlašim | 1:1 (1:0) | ||
| 17:00 | Viktoria Žižkov | - | SFC Opava | 2:2 (1:1) | ||
| 17:00 | Sparta Praag B | - | Vysočina Jihlava | 1:1 (1:0) | ||
| 19:00 | Zbrojovka Brno | - | FK Fotbal Třinec | 4:2 (2:1) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Zbrojovka Brno | 26 | 20 | 2 | 4 | 54:23 | 31 | 62 |
| 2 |
|
FC Sellier & Bellot Vlašim | 26 | 14 | 4 | 8 | 52:34 | 18 | 46 |
| 3 |
|
Sparta Praag B | 26 | 13 | 5 | 8 | 45:30 | 15 | 44 |
| 4 |
|
SK Líšeň | 26 | 12 | 8 | 6 | 40:28 | 12 | 44 |
| 5 |
|
SFC Opava | 26 | 11 | 9 | 6 | 39:28 | 11 | 42 |
| 6 |
|
FK Varnsdorf | 26 | 11 | 5 | 10 | 40:32 | 8 | 38 |
| 7 |
|
FK Dukla Praha | 26 | 10 | 6 | 10 | 35:35 | 0 | 36 |
| 8 |
|
Vysočina Jihlava | 26 | 9 | 8 | 9 | 22:28 | -6 | 35 |
| 9 |
|
FK MAS Táborsko | 26 | 8 | 9 | 9 | 28:31 | -3 | 33 |
| 10 |
|
1. SK Prostějov | 26 | 10 | 3 | 13 | 27:41 | -14 | 33 |
| 11 |
|
1. FK Příbram | 26 | 8 | 8 | 10 | 32:40 | -8 | 32 |
| 12 |
|
MFK Vyškov | 26 | 8 | 6 | 12 | 40:41 | -1 | 30 |
| 13 |
|
MFK Chrudim | 26 | 7 | 9 | 10 | 24:32 | -8 | 30 |
| 14 |
|
FK Fotbal Třinec | 26 | 7 | 6 | 13 | 30:46 | -16 | 27 |
| 15 |
|
FK Ústí nad Labem | 26 | 5 | 10 | 11 | 23:39 | -16 | 25 |
| 16 |
|
Viktoria Žižkov | 26 | 2 | 8 | 16 | 22:45 | -23 | 14 |
| Promotie | |
| Play-offs | |
| Degradatie |
