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| 06.03.2001 | 20:30 | Wrexham AFC | - | Oxford United | 5:3 | |
| 20:45 | Bristol City | - | Walsall FC | 1:3 | ||
| 20:45 | Bury FC | - | Millwall FC | 2:1 | ||
| 20:45 | Luton Town | - | Cambridge United | 1:0 | ||
| 20:45 | Northampton Town | - | Bristol Rovers | 2:1 | ||
| 20:45 | Peterborough United | - | Brentford FC | 1:1 | ||
| 20:45 | Rotherham United | - | AFC Bournemouth | 3:1 | ||
| 20:45 | Swindon Town | - | Oldham Athletic | 3:0 | ||
| 20:45 | Wigan Athletic | - | Notts County | 1:1 | ||
| 20:45 | Wycombe Wanderers | - | Reading FC | 1:1 | ||
| 07.03.2001 | 20:45 | Colchester United | - | Port Vale FC | 0:1 | |
| 20:45 | Stoke City | - | Swansea City | 1:2 |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Rotherham United | 36 | 21 | 8 | 7 | 60:40 | 20 | 71 |
| 2 |
|
Millwall FC | 36 | 21 | 7 | 8 | 68:34 | 34 | 70 |
| 3 |
|
Reading FC | 36 | 20 | 8 | 8 | 67:38 | 29 | 68 |
| 4 |
|
Walsall FC | 36 | 18 | 9 | 9 | 62:41 | 21 | 63 |
| 5 |
|
Stoke City | 36 | 16 | 12 | 8 | 55:36 | 19 | 60 |
| 6 |
|
Wigan Athletic | 36 | 15 | 15 | 6 | 43:31 | 12 | 60 |
| 7 |
|
Bristol City | 36 | 14 | 12 | 10 | 57:42 | 15 | 54 |
| 8 |
|
Notts County | 36 | 15 | 9 | 12 | 46:49 | -3 | 54 |
| 9 |
|
Northampton Town | 36 | 14 | 10 | 12 | 41:44 | -3 | 52 |
| 10 |
|
Bury FC | 36 | 15 | 7 | 14 | 38:43 | -5 | 52 |
| 11 |
|
Port Vale FC | 36 | 13 | 10 | 13 | 42:40 | 2 | 49 |
| 12 |
|
Wrexham AFC | 36 | 13 | 10 | 13 | 52:55 | -3 | 49 |
| 13 |
|
AFC Bournemouth | 36 | 12 | 11 | 13 | 58:50 | 8 | 47 |
| 14 |
|
Peterborough United | 36 | 12 | 11 | 13 | 44:47 | -3 | 47 |
| 15 |
|
Wycombe Wanderers | 36 | 12 | 10 | 14 | 35:39 | -4 | 46 |
| 16 |
|
Brentford FC | 36 | 11 | 13 | 12 | 38:53 | -15 | 46 |
| 17 |
|
Oldham Athletic | 36 | 11 | 11 | 14 | 42:50 | -8 | 44 |
| 18 |
|
Colchester United | 36 | 11 | 9 | 16 | 40:45 | -5 | 42 |
| 19 |
|
Swindon Town | 36 | 11 | 9 | 16 | 36:51 | -15 | 42 |
| 20 |
|
Cambridge United | 36 | 11 | 8 | 17 | 45:56 | -11 | 41 |
| 21 |
|
Luton Town | 36 | 9 | 9 | 18 | 43:62 | -19 | 36 |
| 22 |
|
Bristol Rovers | 36 | 7 | 14 | 15 | 36:45 | -9 | 35 |
| 23 |
|
Swansea City | 36 | 8 | 9 | 19 | 42:59 | -17 | 33 |
| 24 |
|
Oxford United | 36 | 4 | 5 | 27 | 39:79 | -40 | 17 |
| Promotie | |
| Play-offs | |
| Degradatie |
