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| 08.02.2010 | 20:00 | TOP Oss | - | Go Ahead Eagles | 0:0 (0:0) | |
| 20:00 | SBV Excelsior | - | FC Volendam | 4:1 (0:1) | ||
| 20:00 | MVV Maastricht | - | FC Den Bosch | 2:2 (1:1) | ||
| 20:00 | Telstar | - | De Graafschap | 0:2 (0:2) | ||
| 20:00 | FC Dordrecht | - | FC Eindhoven | 0:3 (0:1) | ||
| 20:00 | FC Omniworld | - | FC Emmen | 3:1 (2:1) | ||
| 20:00 | FC Zwolle | - | RBC Roosendaal | 6:1 (1:1) | ||
| 01.03.2010 | 20:00 | SC Cambuur | - | Fortuna Sittard | 2:1 (1:0) | |
| 25.03.2010 | 19:00 | BV Veendam | - | AGOVV | 3:0 (2:0) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 22 | 14 | 5 | 3 | 42:18 | 24 | 47 |
| 2 |
|
Go Ahead Eagles | 22 | 13 | 5 | 4 | 37:17 | 20 | 44 |
| 3 |
|
SC Cambuur | 21 | 12 | 7 | 2 | 48:31 | 17 | 43 |
| 4 |
|
SBV Excelsior | 22 | 12 | 4 | 6 | 42:27 | 15 | 40 |
| 5 |
|
FC Zwolle | 22 | 11 | 5 | 6 | 37:26 | 11 | 38 |
| 6 |
|
FC Den Bosch | 22 | 9 | 8 | 5 | 44:28 | 16 | 35 |
| 7 |
|
Helmond Sport | 21 | 9 | 5 | 7 | 43:36 | 7 | 32 |
| 8 |
|
BV Veendam | 22 | 9 | 6 | 7 | 36:30 | 6 | 32 |
| 9 |
|
FC Dordrecht | 22 | 10 | 2 | 10 | 42:37 | 5 | 32 |
| 10 |
|
AGOVV | 22 | 9 | 6 | 7 | 37:34 | 3 | 31 |
| 11 |
|
RBC Roosendaal | 22 | 8 | 5 | 9 | 28:32 | -4 | 29 |
| 12 |
|
MVV Maastricht | 22 | 9 | 3 | 10 | 40:45 | -5 | 29 |
| 13 |
|
FC Eindhoven | 22 | 7 | 5 | 10 | 41:52 | -11 | 26 |
| 14 |
|
FC Volendam | 21 | 6 | 6 | 9 | 41:47 | -6 | 24 |
| 15 |
|
Telstar | 22 | 5 | 5 | 12 | 30:42 | -12 | 20 |
| 16 |
|
FC Omniworld | 22 | 5 | 4 | 13 | 23:43 | -20 | 19 |
| 17 |
|
FC Emmen | 22 | 5 | 3 | 14 | 25:51 | -26 | 18 |
| 18 |
|
Fortuna Sittard | 22 | 4 | 5 | 13 | 18:38 | -20 | 17 |
| 19 |
|
TOP Oss | 21 | 3 | 5 | 13 | 24:44 | -20 | 14 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotie | |
| Play-offs | |
| Play-offs |
