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| 21.04.2000 | 17:00 | FK Jablonec 97 | - | Slavia Praag | 0:0 (0:0) | |
| 22.04.2000 | 10:15 | Viktoria Žižkov | - | FC Petra Drnovice | 2:0 (0:0) | |
| 16:30 | Dynamo České Budějovice | - | SK Hradec Králové | 2:1 (1:0) | ||
| 16:30 | FC Dukla Pribram | - | CU Bohemians Praha | 3:0 (0:0) | ||
| 16:30 | SFC Opava | - | Sigma Olomouc | 3:0 (1:0) | ||
| 16:30 | Sparta Praag | - | Slovan Liberec | 2:1 (0:0) | ||
| 16:30 | FC Boby-sport Brno | - | Baník Ostrava | 2:1 (1:0) | ||
| 19:00 | FK Teplice | - | Chmel Blsany | 0:0 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 26 | 21 | 3 | 2 | 66:20 | 46 | 66 |
| 2 |
|
Slavia Praag | 26 | 20 | 5 | 1 | 47:15 | 32 | 65 |
| 3 |
|
FC Petra Drnovice | 26 | 14 | 4 | 8 | 34:24 | 10 | 46 |
| 4 |
|
FC Boby-sport Brno | 26 | 10 | 6 | 10 | 29:30 | -1 | 36 |
| 5 |
|
CU Bohemians Praha | 26 | 9 | 8 | 9 | 21:24 | -3 | 35 |
| 6 |
|
FK Teplice | 26 | 8 | 9 | 9 | 34:36 | -2 | 33 |
| 7 |
|
Slovan Liberec | 26 | 7 | 11 | 8 | 17:18 | -1 | 32 |
| 8 |
|
Chmel Blsany | 26 | 9 | 5 | 12 | 23:38 | -15 | 32 |
| 9 |
|
Viktoria Žižkov | 26 | 8 | 7 | 11 | 35:40 | -5 | 31 |
| 10 |
|
Dynamo České Budějovice | 26 | 9 | 4 | 13 | 30:39 | -9 | 31 |
| 11 |
|
FC Dukla Pribram | 26 | 8 | 6 | 12 | 28:35 | -7 | 30 |
| 12 |
|
Sigma Olomouc | 26 | 6 | 11 | 9 | 27:34 | -7 | 29 |
| 13 |
|
Baník Ostrava | 26 | 6 | 10 | 10 | 36:41 | -5 | 28 |
| 14 |
|
SFC Opava | 26 | 6 | 9 | 11 | 30:32 | -2 | 27 |
| 15 |
|
FK Jablonec 97 | 26 | 5 | 9 | 12 | 19:36 | -17 | 24 |
| 16 |
|
SK Hradec Králové | 26 | 3 | 11 | 12 | 19:33 | -14 | 20 |
