| 2. Ronde | 15.08.2009 | Grimsby Town | - | Crewe Alexandra | 0:4 | ||||
| 3. Ronde | 18.08.2009 | Grimsby Town | - | Rotherham United | 1:2 | ||||
| 4. Ronde | 22.08.2009 | Bury FC | - | Grimsby Town | 0:1 | ||||
| 5. Ronde | 29.08.2009 | Grimsby Town | - | Aldershot Town | 1:2 | ||||
| 6. Ronde | 05.09.2009 | Port Vale FC | - | Grimsby Town | 4:0 | ||||
| 7. Ronde | 12.09.2009 | Grimsby Town | - | Hereford United | 1:0 |  |
|||
| 8. Ronde | 19.09.2009 | Torquay United | - | Grimsby Town | 0:2 | ||||
| 9. Ronde | 26.09.2009 | Grimsby Town | - | Darlington FC | 1:1 | ||||
| 11. Ronde | 03.10.2009 | Barnet FC | - | Grimsby Town | 3:0 |  |
|||
| 12. Ronde | 10.10.2009 | Grimsby Town | - | Burton Albion | 1:2 | |
|||
| 13. Ronde | 17.10.2009 | Grimsby Town | - | Rochdale AFC | 0:2 | ||||
| 15. Ronde | 30.10.2009 | Grimsby Town | - | Accrington Stanley | 2:2 | ||||
| 16. Ronde | 14.11.2009 | Northampton Town | - | Grimsby Town | 0:0 | ||||
| 17. Ronde | 21.11.2009 | Lincoln City | - | Grimsby Town | 0:0 | ||||
| 20. Ronde | 05.12.2009 | Grimsby Town | - | Dagenham & Redbridge | 1:1 | ||||
| 21. Ronde | 12.12.2009 | Shrewsbury Town | - | Grimsby Town | 0:0 | ||||
| 27. Ronde | 16.01.2010 | Grimsby Town | - | Cheltenham Town | 0:0 | |
Engeland