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| 08.04.2022 | 16:30 | FK MAS Táborsko | - | SFC Opava | 2:2 (2:2) | |
| 09.04.2022 | 15:30 | Vysočina Jihlava | - | FK Fotbal Třinec | 0:0 (0:0) | |
| 16:00 | SK Líšeň | - | MFK Chrudim | 0:0 (0:0) | ||
| 16:30 | 1. FK Příbram | - | FK Dukla Praha | 2:3 (1:1) | ||
| 16:30 | 1. SK Prostějov | - | FC Sellier & Bellot Vlašim | 3:2 (1:0) | ||
| 10.04.2022 | 10:15 | Viktoria Žižkov | - | Zbrojovka Brno | 0:3 (0:1) | |
| 10:30 | Sparta Praag B | - | MFK Vyškov | 4:1 (0:1) | ||
| 16:30 | FK Varnsdorf | - | FK Ústí nad Labem | 3:0 (0:0) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Zbrojovka Brno | 22 | 17 | 2 | 3 | 46:19 | 27 | 53 |
| 2 |
|
FC Sellier & Bellot Vlašim | 22 | 13 | 2 | 7 | 46:30 | 16 | 41 |
| 3 |
|
SK Líšeň | 22 | 11 | 6 | 5 | 37:25 | 12 | 39 |
| 4 |
|
Sparta Praag B | 22 | 12 | 2 | 8 | 40:26 | 14 | 38 |
| 5 |
|
SFC Opava | 22 | 10 | 6 | 6 | 33:23 | 10 | 36 |
| 6 |
|
FK Varnsdorf | 22 | 10 | 4 | 8 | 37:28 | 9 | 34 |
| 7 |
|
FK Dukla Praha | 22 | 9 | 5 | 8 | 30:30 | 0 | 32 |
| 8 |
|
1. SK Prostějov | 22 | 9 | 3 | 10 | 23:35 | -12 | 30 |
| 9 |
|
MFK Vyškov | 22 | 8 | 4 | 10 | 38:36 | 2 | 28 |
| 10 |
|
FK MAS Táborsko | 22 | 7 | 7 | 8 | 24:27 | -3 | 28 |
| 11 |
|
Vysočina Jihlava | 22 | 7 | 6 | 9 | 18:26 | -8 | 27 |
| 12 |
|
1. FK Příbram | 22 | 6 | 6 | 10 | 28:38 | -10 | 24 |
| 13 |
|
FK Fotbal Třinec | 22 | 7 | 3 | 12 | 23:37 | -14 | 24 |
| 14 |
|
FK Ústí nad Labem | 22 | 5 | 8 | 9 | 20:32 | -12 | 23 |
| 15 |
|
MFK Chrudim | 22 | 5 | 7 | 10 | 21:31 | -10 | 22 |
| 16 |
|
Viktoria Žižkov | 22 | 2 | 5 | 15 | 17:38 | -21 | 11 |
| Promotie | |
| Play-offs | |
| Degradatie |
