| 2. Ronde | 05.09.2021 | Unionistas CF | - | Cultural Leonesa | 2:2 |  |
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| 3. Ronde | 12.09.2021 | Cultural Leonesa | - | Athletic Club B | 2:1 | ||||
| 5. Ronde | 25.09.2021 | Cultural Leonesa | - | Real Valladolid B | 4:2 | ||||
| 10. Ronde | 30.10.2021 | CD Tudelano | - | Cultural Leonesa | 1:1 | ||||
| 11. Ronde | 07.11.2021 | Cultural Leonesa | - | Racing Santander | 3:0 | ||||
| 12. Ronde | 12.11.2021 | SD Logroñés | - | Cultural Leonesa | 1:1 |  |
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| 13. Ronde | 20.11.2021 | Cultural Leonesa | - | UD SS Reyes | 2:0 | |
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| 14. Ronde | 28.11.2021 | CD Calahorra | - | Cultural Leonesa | 2:0 | ||||
| 15. Ronde | 05.12.2021 | Cultural Leonesa | - | Celta de Vigo B | 0:1 | ||||
| 16. Ronde | 11.12.2021 | Zamora CF | - | Cultural Leonesa | 2:2 | ||||
| 19. Ronde | 22.01.2022 | Cultural Leonesa | - | Real Unión | 1:1 | |
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| 20. Ronde | 25.01.2022 | Athletic Club B | - | Cultural Leonesa | 1:2 | |
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| 21. Ronde | 29.01.2022 | Cultural Leonesa | - | CD Badajoz | 1:0 | |
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| 22. Ronde | 09.03.2022 | Racing Santander | - | Cultural Leonesa | 4:0 | |
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| 35. Ronde | 08.05.2022 | Celta de Vigo B | - | Cultural Leonesa | 5:3 | |
Spanje