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| 03.03.2001 | 20:00 | ADO Den Haag | - | BV Emmen | 3:2 (1:1) | |
| 20:00 | FC Den Bosch | - | Go Ahead Eagles | 1:1 (0:0) | ||
| 20:00 | MVV Maastricht | - | SC Cambuur | 3:1 (3:0) | ||
| 20:00 | VVV | - | Dordrecht '90 | 0:1 (0:0) | ||
| 20:00 | SBV Excelsior | - | FC Volendam | 2:1 (1:0) | ||
| 20:00 | BV Veendam | - | Helmond Sport | 4:4 (1:2) | ||
| 04.03.2001 | 14:30 | Telstar | - | HFC Haarlem | 0:1 (0:0) | |
| 05.03.2001 | 20:00 | FC Zwolle | - | Heracles Almelo | 3:1 (2:0) | |
| 20:00 | SBV Eindhoven | - | TOP Oss | 2:0 (0:0) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Den Bosch | 23 | 17 | 2 | 4 | 47:15 | 32 | 53 |
| 2 |
|
SBV Excelsior | 23 | 15 | 4 | 4 | 61:39 | 22 | 49 |
| 3 |
|
FC Zwolle | 23 | 11 | 8 | 4 | 52:30 | 22 | 41 |
| 4 |
|
Go Ahead Eagles | 23 | 12 | 4 | 7 | 45:41 | 4 | 40 |
| 5 |
|
FC Volendam | 23 | 12 | 3 | 8 | 38:30 | 8 | 39 |
| 6 |
|
Helmond Sport | 23 | 10 | 7 | 6 | 52:30 | 22 | 37 |
| 7 |
|
Telstar | 23 | 10 | 5 | 8 | 32:34 | -2 | 35 |
| 8 |
|
BV Emmen | 23 | 10 | 4 | 9 | 43:39 | 4 | 34 |
| 9 |
|
SC Cambuur | 23 | 8 | 8 | 7 | 35:35 | 0 | 32 |
| 10 |
|
Heracles Almelo | 23 | 8 | 6 | 9 | 27:35 | -8 | 30 |
| 11 |
|
MVV Maastricht | 23 | 8 | 3 | 12 | 29:37 | -8 | 27 |
| 12 |
|
ADO Den Haag | 23 | 7 | 6 | 10 | 40:50 | -10 | 27 |
| 13 |
|
SBV Eindhoven | 23 | 7 | 5 | 11 | 34:43 | -9 | 26 |
| 14 |
|
TOP Oss | 23 | 8 | 2 | 13 | 34:46 | -12 | 26 |
| 15 |
|
BV Veendam | 23 | 7 | 3 | 13 | 37:55 | -18 | 24 |
| 16 |
|
Dordrecht '90 | 23 | 4 | 8 | 11 | 33:43 | -10 | 20 |
| 17 |
|
HFC Haarlem | 23 | 4 | 8 | 11 | 28:49 | -21 | 20 |
| 18 |
|
VVV | 23 | 5 | 2 | 16 | 22:38 | -16 | 17 |
