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| 13.03.2009 | 20:00 | Telstar | - | SC Cambuur | 1:1 (1:1) | |
| 20:00 | RBC Roosendaal | - | TOP Oss | 3:2 (2:1) | ||
| 20:00 | FC Dordrecht | - | SBV Excelsior | 0:1 (0:0) | ||
| 20:00 | VVV-Venlo | - | Helmond Sport | 2:0 (2:0) | ||
| 20:00 | AGOVV | - | Fortuna Sittard | 1:0 (1:0) | ||
| 20:00 | MVV Maastricht | - | HFC Haarlem | 3:0 (1:0) | ||
| 20:00 | FC Eindhoven | - | RKC Waalwijk | 1:1 (0:1) | ||
| 20:00 | FC Den Bosch | - | Go Ahead Eagles | 1:0 (0:0) | ||
| 20:00 | FC Zwolle | - | FC Omniworld | 3:1 (1:0) | ||
| 14.03.2009 | 19:30 | FC Emmen | - | BV Veendam | 3:2 (2:1) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
VVV-Venlo | 30 | 20 | 4 | 6 | 70:33 | 37 | 64 |
| 2 |
|
RKC Waalwijk | 30 | 13 | 16 | 1 | 50:26 | 24 | 55 |
| 3 |
|
SC Cambuur | 30 | 15 | 7 | 8 | 54:42 | 12 | 52 |
| 4 |
|
SBV Excelsior | 30 | 13 | 11 | 6 | 46:30 | 16 | 50 |
| 5 |
|
MVV Maastricht | 30 | 12 | 11 | 7 | 46:32 | 14 | 47 |
| 6 |
|
FC Zwolle | 30 | 13 | 7 | 10 | 48:44 | 4 | 46 |
| 7 |
|
FC Den Bosch | 30 | 12 | 8 | 10 | 53:37 | 16 | 44 |
| 8 |
|
Helmond Sport | 30 | 11 | 10 | 9 | 44:40 | 4 | 43 |
| 9 |
|
HFC Haarlem | 30 | 13 | 4 | 13 | 36:44 | -8 | 43 |
| 10 |
|
Go Ahead Eagles | 30 | 10 | 12 | 8 | 29:33 | -4 | 42 |
| 11 |
|
TOP Oss | 30 | 11 | 6 | 13 | 41:48 | -7 | 39 |
| 12 |
|
AGOVV | 30 | 11 | 5 | 14 | 45:54 | -9 | 38 |
| 13 |
|
RBC Roosendaal | 30 | 10 | 7 | 13 | 36:38 | -2 | 37 |
| 14 |
|
Telstar | 30 | 8 | 11 | 11 | 38:45 | -7 | 35 |
| 15 |
|
FC Emmen | 30 | 9 | 7 | 14 | 38:56 | -18 | 34 |
| 16 |
|
Fortuna Sittard | 30 | 8 | 8 | 14 | 30:46 | -16 | 32 |
| 17 |
|
FC Dordrecht | 30 | 7 | 10 | 13 | 31:34 | -3 | 31 |
| 18 |
|
FC Eindhoven | 30 | 6 | 13 | 11 | 48:65 | -17 | 28 |
| 19 |
|
BV Veendam | 30 | 7 | 8 | 15 | 37:54 | -17 | 26 |
| 20 |
|
FC Omniworld | 30 | 6 | 5 | 19 | 33:52 | -19 | 23 |
| Promotie | |
| Play-offs | |
| Play-offs | |
| Play-offs | |
| Play-offs |
