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| 16.04.2010 | 20:00 | FC Emmen | - | FC Zwolle | 0:3 (0:2) | |
| 20:00 | AGOVV | - | FC Dordrecht | 0:2 (0:0) | ||
| 20:00 | TOP Oss | - | Telstar | 1:1 (1:1) | ||
| 20:00 | FC Eindhoven | - | De Graafschap | 2:6 (2:1) | ||
| 20:00 | FC Volendam | - | FC Omniworld | 3:0 (1:0) | ||
| 20:00 | SBV Excelsior | - | FC Den Bosch | 5:1 (2:0) | ||
| 20:00 | Go Ahead Eagles | - | RBC Roosendaal | 3:1 (2:1) | ||
| 20:00 | SC Cambuur | - | Helmond Sport | 2:0 (1:0) | ||
| 20:00 | Fortuna Sittard | - | BV Veendam | 0:1 (0:1) |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
De Graafschap | 35 | 25 | 6 | 4 | 82:29 | 53 | 81 |
| 2 |
|
SC Cambuur | 35 | 21 | 8 | 6 | 78:47 | 31 | 71 |
| 3 |
|
SBV Excelsior | 35 | 19 | 5 | 11 | 72:47 | 25 | 62 |
| 4 |
|
Go Ahead Eagles | 35 | 18 | 8 | 9 | 52:32 | 20 | 62 |
| 5 |
|
FC Zwolle | 35 | 18 | 8 | 9 | 56:37 | 19 | 62 |
| 6 |
|
FC Den Bosch | 36 | 14 | 12 | 10 | 66:54 | 12 | 54 |
| 7 |
|
AGOVV | 35 | 15 | 9 | 11 | 58:49 | 9 | 52 |
| 8 |
|
BV Veendam | 35 | 14 | 10 | 11 | 56:47 | 9 | 51 |
| 9 |
|
Helmond Sport | 35 | 15 | 6 | 14 | 61:53 | 8 | 51 |
| 10 |
|
MVV Maastricht | 35 | 13 | 7 | 15 | 54:66 | -12 | 45 |
| 11 |
|
RBC Roosendaal | 35 | 11 | 10 | 14 | 46:53 | -7 | 43 |
| 12 |
|
FC Eindhoven | 35 | 12 | 7 | 16 | 61:80 | -19 | 43 |
| 13 |
|
FC Dordrecht | 35 | 12 | 4 | 19 | 51:57 | -6 | 40 |
| 14 |
|
FC Omniworld | 35 | 11 | 7 | 17 | 38:60 | -22 | 40 |
| 15 |
|
FC Emmen | 35 | 10 | 8 | 17 | 51:77 | -26 | 38 |
| 16 |
|
FC Volendam | 35 | 8 | 11 | 16 | 59:78 | -19 | 35 |
| 17 |
|
Fortuna Sittard | 35 | 7 | 9 | 19 | 31:54 | -23 | 30 |
| 18 |
|
Telstar | 35 | 7 | 8 | 20 | 44:63 | -19 | 29 |
| 19 |
|
TOP Oss | 35 | 6 | 11 | 18 | 41:74 | -33 | 29 |
| 20 |
|
HFC Haarlem | 0 | 0 | 0 | 0 | 0:0 | 0 | 0 |
| Promotie | |
| Play-offs | |
| Play-offs |
