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| 26.08.2006 | 14:00 | VV Alkmaarsche Boys | - | Flevo Boys | 0:1 (0:1) | |
| 14:30 | ASWH | - | WSV Apeldoorn | 5:1 (2:1) | ||
| 14:30 | DOTO Pernis | - | GVVV | 1:0 (0:0) | ||
| 14:30 | Excelsior '31 | - | VV Geldrop/AEK | 3:1 (0:0) | ||
| 14:30 | Harkemase Boys | - | SV Argon | 0:4 (0:0) | ||
| 14:30 | FC Lisse | - | EVV | 3:5 (1:0, 1:1, 2:2) d.p. | ||
| 14:30 | ONS Sneek | - | VV Appingedam | 4:0 (3:0) | ||
| 14:30 | Ter Leede | - | FC Omniworld | 4:2 (1:1) | ||
| 14:30 | WHC Wezep | - | Gemert | 2:0 (1:0) | ||
| 14:30 | RKSV Oisterwijk | - | HHC Hardenberg | 0:2 (0:1) | ||
| 15:00 | VV Bennekom | - | ADO '20 | 1:0 (1:0) | ||
| 15:00 | Sparta Nijkerk | - | USV Elinkwijk | 2:1 (1:1) | ||
| 15:00 | IJsselmeervogels | - | JVC Cuijk | 3:0 (3:0) | ||
| 17:00 | SV Babberich | - | Rijnsburgse Boys | 4:5 (0:0, 1:1, 1:1) d.p. | ||
| 17:00 | FC Türkiyemspor Amsterdam | - | Spakenburg | 5:1 (2:1) | ||
| 17:00 | Kozakken Boys | - | Roda Boys | 3:0 (2:0) | ||
| 18:00 | HSC'21 | - | Spijkenisse | 3:0 (2:0) | ||
| 18:00 | SV Meerssen | - | Quick Boys | 7:6 (1:0, 1:1, 1:1) d.p. | ||
| 18:00 | RKSV Schijndel | - | ACV Assen | 2:0 (1:0) | ||
| 18:00 | De Treffers | - | Katwijk | 3:2 (1:1, 2:2) n.v. | ||
| 18:00 | UDI'19/CSU | - | SC Genemuiden | 0:1 (0:0) | ||
| 18:00 | FC Zutphen | - | Achilles'29 | 0:4 (0:0) | ||
| 19:30 | VV Caesar | - | VVOG | 8:9 (0:0, 0:0, 0:0) d.p. | ||
| 19:30 | VSV Tonegido | - | Be Quick '28 | 0:5 (0:3) |
