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| 21.10.2023 | 15:00 | FC Zlín | - | FK Mladá Boleslav | 5:9 (2:1) | |
| 15:00 | FK Pardubice | - | Sigma Olomouc | 1:1 (1:0) | ||
| 15:00 | MFK Karviná | - | FC Hradec Králové | 1:0 (0:0) | ||
| 15:00 | Slovan Liberec | - | Viktoria Plzeň | 3:0 (1:0) | ||
| 18:00 | Slavia Praag | - | 1. FC Slovácko | 2:0 (0:0) | ||
| 22.10.2023 | 15:00 | FK Teplice | - | Baník Ostrava | 0:1 (0:0) | |
| 15:00 | Sparta Praag | - | Dynamo České Budějovice | 4:0 (2:0) | ||
| 18:00 | FK Jablonec | - | Bohemians Praha 1905 | 0:1 (0:1) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 12 | 10 | 2 | 0 | 34:8 | 26 | 32 |
| 2 |
|
Slavia Praag | 12 | 9 | 3 | 0 | 22:7 | 15 | 30 |
| 3 |
|
Viktoria Plzeň | 12 | 8 | 1 | 3 | 33:17 | 16 | 25 |
| 4 |
|
Sigma Olomouc | 12 | 7 | 1 | 4 | 22:15 | 7 | 22 |
| 5 |
|
Baník Ostrava | 12 | 6 | 3 | 3 | 19:10 | 9 | 21 |
| 6 |
|
FK Mladá Boleslav | 12 | 6 | 2 | 4 | 28:24 | 4 | 20 |
| 7 |
|
1. FC Slovácko | 12 | 6 | 2 | 4 | 13:14 | -1 | 20 |
| 8 |
|
Bohemians Praha 1905 | 12 | 5 | 2 | 5 | 11:14 | -3 | 17 |
| 9 |
|
Slovan Liberec | 12 | 3 | 4 | 5 | 18:21 | -3 | 13 |
| 10 |
|
FK Teplice | 12 | 3 | 4 | 5 | 10:13 | -3 | 13 |
| 11 |
|
FC Hradec Králové | 12 | 3 | 2 | 7 | 14:21 | -7 | 11 |
| 12 |
|
MFK Karviná | 12 | 3 | 1 | 8 | 16:23 | -7 | 10 |
| 13 |
|
FK Pardubice | 12 | 2 | 4 | 6 | 10:19 | -9 | 10 |
| 14 |
|
Dynamo České Budějovice | 12 | 3 | 1 | 8 | 14:29 | -15 | 10 |
| 15 |
|
FK Jablonec | 12 | 1 | 5 | 6 | 10:18 | -8 | 8 |
| 16 |
|
FC Zlín | 12 | 1 | 3 | 8 | 13:34 | -21 | 6 |
| Play-offs | |
