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| 24.03.2000 | 17:00 | FK Teplice | - | CU Bohemians Praha | 2:0 (1:0) | |
| 25.03.2000 | 10:15 | Viktoria Žižkov | - | Slovan Liberec | 0:0 (0:0) | |
| 15:00 | FC Dukla Pribram | - | Dynamo České Budějovice | 1:0 (0:0) | ||
| 15:00 | FK Jablonec 97 | - | Baník Ostrava | 2:2 (1:1) | ||
| 15:00 | SFC Opava | - | SK Hradec Králové | 0:0 (0:0) | ||
| 15:00 | Sparta Praag | - | FC Boby-sport Brno | 3:0 (0:0) | ||
| 16:30 | FC Petra Drnovice | - | Chmel Blsany | 2:0 (1:0) | ||
| 26.03.2000 | 16:30 | Sigma Olomouc | - | Slavia Praag | 0:2 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Slavia Praag | 22 | 18 | 4 | 0 | 41:10 | 31 | 58 |
| 2 |
|
Sparta Praag | 22 | 17 | 3 | 2 | 59:19 | 40 | 54 |
| 3 |
|
FC Petra Drnovice | 22 | 12 | 4 | 6 | 30:19 | 11 | 40 |
| 4 |
|
CU Bohemians Praha | 22 | 8 | 7 | 7 | 19:19 | 0 | 31 |
| 5 |
|
FK Teplice | 22 | 8 | 6 | 8 | 31:31 | 0 | 30 |
| 6 |
|
Chmel Blsany | 22 | 9 | 3 | 10 | 22:34 | -12 | 30 |
| 7 |
|
Dynamo České Budějovice | 22 | 8 | 3 | 11 | 26:33 | -7 | 27 |
| 8 |
|
FC Dukla Pribram | 22 | 7 | 6 | 9 | 23:30 | -7 | 27 |
| 9 |
|
Baník Ostrava | 22 | 6 | 8 | 8 | 32:34 | -2 | 26 |
| 10 |
|
FC Boby-sport Brno | 22 | 7 | 5 | 10 | 23:28 | -5 | 26 |
| 11 |
|
Slovan Liberec | 22 | 5 | 10 | 7 | 13:16 | -3 | 25 |
| 12 |
|
SFC Opava | 22 | 5 | 8 | 9 | 26:28 | -2 | 23 |
| 13 |
|
Sigma Olomouc | 22 | 4 | 11 | 7 | 24:30 | -6 | 23 |
| 14 |
|
Viktoria Žižkov | 22 | 5 | 7 | 10 | 28:37 | -9 | 22 |
| 15 |
|
SK Hradec Králové | 22 | 3 | 9 | 10 | 17:28 | -11 | 18 |
| 16 |
|
FK Jablonec 97 | 22 | 4 | 6 | 12 | 17:35 | -18 | 18 |
