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| 06.04.2001 | 16:30 | Sigma Olomouc | - | Slavia Praag | 1:5 (0:1) | |
| 07.04.2001 | 16:30 | FC Stavo Artikel Brno | - | FK Jablonec 97 | 1:0 (1:0) | |
| 08.04.2001 | 10:15 | Viktoria Žižkov | - | Chmel Blsany | 1:0 (1:0) | |
| 16:30 | Baník Ostrava | - | Viktoria Plzeň | 3:0 (2:0) | ||
| 16:30 | FC Marila Příbram | - | Dynamo České Budějovice | 1:0 (1:0) | ||
| 16:30 | Slovan Liberec | - | CU Bohemians Praha | 3:0 (2:0) | ||
| 16:30 | FK Teplice | - | FK Drnovice | 0:1 (0:1) | ||
| 09.04.2001 | 19:00 | Sparta Praag | - | 1. FC Synot Stare Mesto | 2:0 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 23 | 17 | 3 | 3 | 57:22 | 35 | 54 |
| 2 |
|
FC Marila Příbram | 23 | 11 | 6 | 6 | 33:22 | 11 | 39 |
| 3 |
|
Sigma Olomouc | 23 | 10 | 8 | 5 | 39:27 | 12 | 38 |
| 4 |
|
Viktoria Žižkov | 23 | 10 | 7 | 6 | 36:33 | 3 | 37 |
| 5 |
|
Slavia Praag | 23 | 9 | 9 | 5 | 36:27 | 9 | 36 |
| 6 |
|
Slovan Liberec | 23 | 10 | 5 | 8 | 34:28 | 6 | 35 |
| 7 |
|
FK Drnovice | 23 | 9 | 6 | 8 | 26:25 | 1 | 33 |
| 8 |
|
FK Teplice | 23 | 10 | 3 | 10 | 35:28 | 7 | 33 |
| 9 |
|
1. FC Synot Stare Mesto | 23 | 7 | 9 | 7 | 30:28 | 2 | 30 |
| 10 |
|
FK Jablonec 97 | 23 | 7 | 7 | 9 | 21:30 | -9 | 28 |
| 11 |
|
CU Bohemians Praha | 23 | 6 | 9 | 8 | 27:31 | -4 | 27 |
| 12 |
|
FC Stavo Artikel Brno | 23 | 7 | 5 | 11 | 20:26 | -6 | 26 |
| 13 |
|
Baník Ostrava | 23 | 6 | 8 | 9 | 25:34 | -9 | 26 |
| 14 |
|
Chmel Blsany | 23 | 5 | 8 | 10 | 20:29 | -9 | 23 |
| 15 |
|
Dynamo České Budějovice | 23 | 4 | 7 | 12 | 27:47 | -20 | 19 |
| 16 |
|
Viktoria Plzeň | 23 | 3 | 6 | 14 | 19:48 | -29 | 15 |
