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| 20.04.2001 | 16:30 | FC Marila Příbram | - | FK Drnovice | 1:1 (0:0) | |
| 21.04.2001 | 10:15 | Viktoria Žižkov | - | Dynamo České Budějovice | 2:0 (1:0) | |
| 14:00 | FC Stavo Artikel Brno | - | Slavia Praag | 0:2 (0:0) | ||
| 16:30 | Baník Ostrava | - | Slovan Liberec | 0:1 (0:1) | ||
| 16:30 | FK Jablonec 97 | - | Chmel Blsany | 0:2 (0:1) | ||
| 16:30 | Sigma Olomouc | - | 1. FC Synot Stare Mesto | 3:2 (2:1) | ||
| 16:30 | Sparta Praag | - | CU Bohemians Praha | 1:0 (0:0) | ||
| 16:30 | FK Teplice | - | Viktoria Plzeň | 5:1 (2:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 25 | 19 | 3 | 3 | 61:24 | 37 | 60 |
| 2 |
|
Sigma Olomouc | 25 | 12 | 8 | 5 | 44:30 | 14 | 44 |
| 3 |
|
FC Marila Příbram | 25 | 11 | 8 | 6 | 34:23 | 11 | 41 |
| 4 |
|
Viktoria Žižkov | 25 | 11 | 8 | 6 | 38:33 | 5 | 41 |
| 5 |
|
Slavia Praag | 25 | 10 | 10 | 5 | 38:27 | 11 | 40 |
| 6 |
|
FK Teplice | 25 | 12 | 3 | 10 | 41:29 | 12 | 39 |
| 7 |
|
Slovan Liberec | 25 | 11 | 5 | 9 | 35:29 | 6 | 38 |
| 8 |
|
FK Drnovice | 25 | 9 | 7 | 9 | 28:28 | 0 | 34 |
| 9 |
|
1. FC Synot Stare Mesto | 25 | 7 | 9 | 9 | 32:32 | 0 | 30 |
| 10 |
|
CU Bohemians Praha | 25 | 7 | 9 | 9 | 29:32 | -3 | 30 |
| 11 |
|
FK Jablonec 97 | 25 | 7 | 8 | 10 | 21:32 | -11 | 29 |
| 12 |
|
Chmel Blsany | 25 | 7 | 8 | 10 | 23:29 | -6 | 29 |
| 13 |
|
FC Stavo Artikel Brno | 25 | 7 | 5 | 13 | 21:30 | -9 | 26 |
| 14 |
|
Baník Ostrava | 25 | 6 | 8 | 11 | 25:37 | -12 | 26 |
| 15 |
|
Dynamo České Budějovice | 25 | 5 | 7 | 13 | 29:50 | -21 | 22 |
| 16 |
|
Viktoria Plzeň | 25 | 3 | 6 | 16 | 22:56 | -34 | 15 |
