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| 06.05.2001 | 10:15 | Viktoria Žižkov | - | FK Drnovice | 3:1 (2:0) | |
| 16:30 | FK Jablonec 97 | - | Slavia Praag | 1:2 (0:1) | ||
| 16:30 | FC Marila Příbram | - | Viktoria Plzeň | 3:1 (1:1) | ||
| 16:30 | Sigma Olomouc | - | CU Bohemians Praha | 1:0 (0:0) | ||
| 16:30 | FC Stavo Artikel Brno | - | 1. FC Synot Stare Mesto | 0:2 (0:0) | ||
| 16:30 | FK Teplice | - | Slovan Liberec | 0:0 (0:0) | ||
| 19:00 | Sparta Praag | - | Baník Ostrava | 4:1 (2:0) | ||
| 07.05.2001 | 19:00 | Dynamo České Budějovice | - | Chmel Blsany | 1:1 (0:1) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 27 | 20 | 4 | 3 | 66:26 | 40 | 64 |
| 2 |
|
Sigma Olomouc | 27 | 13 | 9 | 5 | 46:31 | 15 | 48 |
| 3 |
|
FC Marila Příbram | 27 | 13 | 8 | 6 | 38:24 | 14 | 47 |
| 4 |
|
Slavia Praag | 27 | 12 | 10 | 5 | 41:28 | 13 | 46 |
| 5 |
|
Viktoria Žižkov | 27 | 12 | 8 | 7 | 41:35 | 6 | 44 |
| 6 |
|
Slovan Liberec | 27 | 11 | 7 | 9 | 36:30 | 6 | 40 |
| 7 |
|
FK Teplice | 27 | 12 | 4 | 11 | 42:31 | 11 | 40 |
| 8 |
|
FK Drnovice | 27 | 9 | 8 | 10 | 31:33 | -2 | 35 |
| 9 |
|
1. FC Synot Stare Mesto | 27 | 8 | 9 | 10 | 34:33 | 1 | 33 |
| 10 |
|
CU Bohemians Praha | 27 | 8 | 9 | 10 | 31:34 | -3 | 33 |
| 11 |
|
Chmel Blsany | 27 | 8 | 9 | 10 | 27:30 | -3 | 33 |
| 12 |
|
FK Jablonec 97 | 27 | 7 | 8 | 12 | 23:36 | -13 | 29 |
| 13 |
|
FC Stavo Artikel Brno | 27 | 7 | 6 | 14 | 23:34 | -11 | 27 |
| 14 |
|
Baník Ostrava | 27 | 6 | 8 | 13 | 26:44 | -18 | 26 |
| 15 |
|
Dynamo České Budějovice | 27 | 6 | 8 | 13 | 32:52 | -20 | 26 |
| 16 |
|
Viktoria Plzeň | 27 | 3 | 7 | 17 | 24:60 | -36 | 16 |
