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| 18.05.2001 | 16:30 | Dynamo České Budějovice | - | FK Drnovice | 0:1 (0:1) | |
| 16:30 | FK Jablonec 97 | - | 1. FC Synot Stare Mesto | 1:0 (1:0) | ||
| 16:30 | FC Marila Příbram | - | Slovan Liberec | 1:1 (0:0) | ||
| 16:30 | Sigma Olomouc | - | Baník Ostrava | 0:0 (0:0) | ||
| 16:30 | Slavia Praag | - | Chmel Blsany | 2:3 (1:2) | ||
| 16:30 | FC Stavo Artikel Brno | - | CU Bohemians Praha | 0:0 (0:0) | ||
| 16:30 | FK Teplice | - | Sparta Praag | 2:3 (0:1) | ||
| 16:30 | Viktoria Žižkov | - | Viktoria Plzeň | 3:3 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 29 | 21 | 5 | 3 | 71:30 | 41 | 68 |
| 2 |
|
Slavia Praag | 29 | 13 | 10 | 6 | 44:31 | 13 | 49 |
| 3 |
|
Sigma Olomouc | 29 | 13 | 10 | 6 | 46:33 | 13 | 49 |
| 4 |
|
FC Marila Příbram | 29 | 13 | 9 | 7 | 39:26 | 13 | 48 |
| 5 |
|
Viktoria Žižkov | 29 | 12 | 10 | 7 | 45:39 | 6 | 46 |
| 6 |
|
Slovan Liberec | 29 | 12 | 8 | 9 | 39:31 | 8 | 44 |
| 7 |
|
FK Drnovice | 29 | 11 | 8 | 10 | 34:34 | 0 | 41 |
| 8 |
|
FK Teplice | 29 | 12 | 4 | 13 | 44:36 | 8 | 40 |
| 9 |
|
CU Bohemians Praha | 29 | 9 | 10 | 10 | 32:34 | -2 | 37 |
| 10 |
|
Chmel Blsany | 29 | 9 | 10 | 10 | 32:34 | -2 | 37 |
| 11 |
|
1. FC Synot Stare Mesto | 29 | 8 | 10 | 11 | 35:35 | 0 | 34 |
| 12 |
|
FK Jablonec 97 | 29 | 8 | 8 | 13 | 25:38 | -13 | 32 |
| 13 |
|
Baník Ostrava | 29 | 7 | 9 | 13 | 28:44 | -16 | 30 |
| 14 |
|
FC Stavo Artikel Brno | 29 | 7 | 8 | 14 | 24:35 | -11 | 29 |
| 15 |
|
Dynamo České Budějovice | 29 | 6 | 8 | 15 | 32:54 | -22 | 26 |
| 16 |
|
Viktoria Plzeň | 29 | 3 | 9 | 17 | 28:64 | -36 | 18 |
