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| 17.04.2010 | 15:45 | Viktoria Plzeň | - | FK Baumit Jablonec | 1:1 (1:0) | |
| 18:00 | 1. FC Slovácko | - | Bohemians 1905 | 1:1 (1:0) | ||
| 18:00 | 1. FC Brno | - | Sigma Olomouc | 1:2 (0:1) | ||
| 18.04.2010 | 17:00 | Bohemians Praha | - | FK Mladá Boleslav | 2:4 (0:2) | |
| 17:00 | Dynamo České Budějovice | - | Sparta Praag | 0:0 (0:0) | ||
| 17:00 | Slavia Praag | - | SK Kladno | 0:0 (0:0) | ||
| 19:30 | FK Teplice | - | 1. FK Příbram | 1:0 (1:0) | ||
| 19.04.2010 | 17:30 | Slovan Liberec | - | Baník Ostrava | 0:1 (0:0) |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Sparta Praag | 25 | 13 | 12 | 0 | 33:11 | 22 | 51 |
| 2 |
|
Baník Ostrava | 25 | 15 | 6 | 4 | 39:21 | 18 | 51 |
| 3 |
|
FK Baumit Jablonec | 25 | 14 | 6 | 5 | 32:24 | 8 | 48 |
| 4 |
|
FK Teplice | 25 | 13 | 8 | 4 | 37:23 | 14 | 47 |
| 5 |
|
Viktoria Plzeň | 25 | 11 | 8 | 6 | 32:25 | 7 | 41 |
| 6 |
|
FK Mladá Boleslav | 25 | 11 | 5 | 9 | 45:35 | 10 | 38 |
| 7 |
|
Sigma Olomouc | 25 | 11 | 4 | 10 | 39:32 | 7 | 37 |
| 8 |
|
Slavia Praag | 25 | 9 | 8 | 8 | 30:25 | 5 | 35 |
| 9 |
|
1. FK Příbram | 25 | 9 | 4 | 12 | 30:34 | -4 | 31 |
| 10 |
|
Slovan Liberec | 25 | 9 | 4 | 12 | 28:32 | -4 | 31 |
| 11 |
|
1. FC Brno | 25 | 8 | 6 | 11 | 25:34 | -9 | 30 |
| 12 |
|
Bohemians 1905 | 25 | 7 | 8 | 10 | 18:24 | -6 | 29 |
| 13 |
|
Dynamo České Budějovice | 25 | 5 | 9 | 11 | 20:29 | -9 | 24 |
| 14 |
|
1. FC Slovácko | 25 | 6 | 5 | 14 | 23:34 | -11 | 23 |
| 15 |
|
SK Kladno | 25 | 6 | 3 | 16 | 22:40 | -18 | 21 |
| 16 |
|
Bohemians Praha | 25 | 4 | 2 | 19 | 25:55 | -30 | -6 |
| Champions League Kwali. | |
| Europa League | |
| Degradatie |
