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| U | Go Ahead Eagles | - | SC Cambuur | 2:0 (1:0) | ||
| R | SC Cambuur | - | Go Ahead Eagles | 1:0 (1:0) | ||
| U: 06.05.2010 19:00 | R: 09.05.2010 14:30 | Winnaars: Go Ahead Eagles | ||||
| U | FC Eindhoven | - | Willem II | 1:2 (1:0) | ||
| R | Willem II | - | FC Eindhoven | 1:1 (1:0) | ||
| U: 06.05.2010 20:00 | R: 09.05.2010 14:30 | Winnaars: Willem II | ||||
| U | FC Zwolle | - | SBV Excelsior | 0:1 (0:1) | ||
| R | SBV Excelsior | - | FC Zwolle | 4:3 (2:2) | ||
| U: 06.05.2010 20:00 | R: 09.05.2010 14:30 | Winnaars: SBV Excelsior | ||||
| U | Helmond Sport | - | Sparta Rotterdam | 2:1 (0:0) | ||
| R | Sparta Rotterdam | - | Helmond Sport | 2:0 (0:0) | ||
| U: 06.05.2010 21:15 | R: 09.05.2010 16:30 | Winnaars: Sparta Rotterdam | ||||
