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| U | Guadix Club de Fútbol | - | Granada CF | 0:0 (0:0) | ||
| R | Granada CF | - | Guadix Club de Fútbol | 5:4 (0:0, 0:0, 0:0) d.p. | ||
| U: 10.01.2001 20:30 | R: 17.01.2001 20:30 | Winnaars: Granada CF | ||||
| U | Racing Santander | - | Athletic Bilbao | 2:0 (2:0) | ||
| R | Athletic Bilbao | - | Racing Santander | 0:0 (0:0) | ||
| U: 10.01.2001 20:30 | R: 17.01.2001 20:15 | Winnaars: Racing Santander | ||||
| U | CD Leganés | - | Celta de Vigo | 1:2 (1:1) | ||
| R | Celta de Vigo | - | CD Leganés | 0:1 (0:0) | ||
| U: 10.01.2001 21:00 | R: 17.01.2001 21:15 | Winnaars: Celta de Vigo | ||||
| U | CF Extremadura | - | Espanyol | 1:1 (0:0) | ||
| R | Espanyol | - | CF Extremadura | 1:0 (0:0) | ||
| U: 10.01.2001 21:00 | R: 17.01.2001 21:00 | Winnaars: Espanyol | ||||
| U | Real Murcia | - | Real Zaragoza | 2:3 (1:0) | ||
| R | Real Zaragoza | - | Real Murcia | 4:1 (2:0) | ||
| U: 10.01.2001 21:00 | R: 17.01.2001 20:30 | Winnaars: Real Zaragoza | ||||
| U | Gimnástica Torrelavega | - | FC Barcelona | 0:1 (0:0) | ||
| R | FC Barcelona | - | Gimnástica Torrelavega | 0:0 (0:0) | ||
| U: 10.01.2001 21:15 | R: 16.01.2001 21:30 | Winnaars: FC Barcelona | ||||
| U | CD Tenerife | - | RCD Mallorca | 0:2 (0:2) | ||
| R | RCD Mallorca | - | CD Tenerife | 2:0 (1:0) | ||
| U: 10.01.2001 22:00 | R: 17.01.2001 21:00 | Winnaars: RCD Mallorca | ||||
| U | Atlético Madrid | - | Rayo Vallecano | 2:2 (2:0) | ||
| R | Rayo Vallecano | - | Atlético Madrid | 0:2 (0:1) | ||
| U: 11.01.2001 21:30 | R: 17.01.2001 21:00 | Winnaars: Atlético Madrid | ||||
