| 1. Ronde | 08.08.2009 | Yeovil Town | - | Tranmere Rovers | 2:0 | ||||
| 2. Ronde | 15.08.2009 | Tranmere Rovers | - | Gillingham FC | 4:2 | ||||
| 3. Ronde | 18.08.2009 | Tranmere Rovers | - | Milton Keynes Dons | 0:1 | ||||
| 4. Ronde | 22.08.2009 | Leeds United | - | Tranmere Rovers | 3:0 | ||||
| 5. Ronde | 29.08.2009 | Tranmere Rovers | - | Charlton Athletic | 0:4 | ||||
| 6. Ronde | 05.09.2009 | Carlisle United | - | Tranmere Rovers | 3:0 | ||||
| 7. Ronde | 12.09.2009 | Tranmere Rovers | - | Walsall FC | 2:3 |  |
|||
| 8. Ronde | 19.09.2009 | Exeter City | - | Tranmere Rovers | 2:1 | ||||
| 9. Ronde | 26.09.2009 | Tranmere Rovers | - | Colchester United | 1:1 | ||||
| 10. Ronde | 29.09.2009 | Wycombe Wanderers | - | Tranmere Rovers | 0:1 | ||||
| 11. Ronde | 03.10.2009 | Millwall FC | - | Tranmere Rovers | 5:0 | ||||
| 12. Ronde | 12.10.2009 | Tranmere Rovers | - | Stockport County | 0:1 | ||||
| 29. Ronde | 30.01.2010 | Charlton Athletic | - | Tranmere Rovers | 1:1 | ||||
| 35. Ronde | 06.03.2010 | Tranmere Rovers | - | Southampton FC | 2:1 | ||||
| 25. Ronde | 09.03.2010 | Tranmere Rovers | - | Leeds United | 1:4 | ||||
| 40. Ronde | 05.04.2010 | Swindon Town | - | Tranmere Rovers | 3:0 |
Ierland