| 1. Ronde | 08.08.2009 | Southampton FC | - | Millwall FC | 1:1 | ||||
| 2. Ronde | 15.08.2009 | Huddersfield Town | - | Southampton FC | 3:1 | ||||
| 4. Ronde | 22.08.2009 | Southampton FC | - | Brentford FC | 1:1 | ||||
| 5. Ronde | 29.08.2009 | Stockport County | - | Southampton FC | 1:1 | ||||
| 6. Ronde | 05.09.2009 | Southampton FC | - | Colchester United | 0:0 | ||||
| 7. Ronde | 12.09.2009 | Charlton Athletic | - | Southampton FC | 1:1 | ||||
| 12. Ronde | 09.10.2009 | Southend United | - | Southampton FC | 1:3 | ||||
| 19. Ronde | 01.12.2009 | Southampton FC | - | Wycombe Wanderers | 1:0 | ||||
| 20. Ronde | 05.12.2009 | Walsall FC | - | Southampton FC | 1:3 | ||||
| 21. Ronde | 12.12.2009 | Southampton FC | - | Tranmere Rovers | 3:0 | ||||
| 22. Ronde | 19.12.2009 | Leeds United | - | Southampton FC | 1:0 | ||||
| 23. Ronde | 26.12.2009 | Southampton FC | - | Exeter City | 3:1 |  |
Engeland