| 4. Ronde | 22.08.2009 | Leeds United | - | Tranmere Rovers | 3:0 | ||||
| 5. Ronde | 29.08.2009 | Tranmere Rovers | - | Charlton Athletic | 0:4 | ||||
| 7. Ronde | 12.09.2009 | Tranmere Rovers | - | Walsall FC | 2:3 | ||||
| 8. Ronde | 19.09.2009 | Exeter City | - | Tranmere Rovers | 2:1 |  |
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| 9. Ronde | 26.09.2009 | Tranmere Rovers | - | Colchester United | 1:1 | ||||
| 10. Ronde | 29.09.2009 | Wycombe Wanderers | - | Tranmere Rovers | 0:1 | ||||
| 11. Ronde | 03.10.2009 | Millwall FC | - | Tranmere Rovers | 5:0 | ||||
| 15. Ronde | 31.10.2009 | Tranmere Rovers | - | Swindon Town | 1:4 | ||||
| 16. Ronde | 14.11.2009 | Norwich City | - | Tranmere Rovers | 2:0 | ||||
| 17. Ronde | 21.11.2009 | Leyton Orient | - | Tranmere Rovers | 2:1 | ||||
| 19. Ronde | 01.12.2009 | Huddersfield Town | - | Tranmere Rovers | 3:3 | |
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| 21. Ronde | 12.12.2009 | Southampton FC | - | Tranmere Rovers | 3:0 |
Engeland