| 30. Ronde | 05.02.2022 | Leyton Orient | - | Colchester United | 0:1 | ||||
| 31. Ronde | 08.02.2022 | Mansfield Town | - | Colchester United | 2:1 | ||||
| 32. Ronde | 12.02.2022 | Colchester United | - | Carlisle United | 2:2 |  |
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| 33. Ronde | 19.02.2022 | Northampton Town | - | Colchester United | 3:0 | ||||
| 22. Ronde | 22.02.2022 | Colchester United | - | Hartlepool United | 1:2 | ||||
| 34. Ronde | 26.02.2022 | Colchester United | - | Oldham Athletic | 1:1 | ||||
| 35. Ronde | 05.03.2022 | Colchester United | - | Port Vale FC | 1:0 | ||||
| 36. Ronde | 12.03.2022 | Scunthorpe United | - | Colchester United | 1:3 | ||||
| 38. Ronde | 21.03.2022 | Colchester United | - | Forest Green Rovers | 0:1 | ||||
| 41. Ronde | 09.04.2022 | Colchester United | - | Stevenage FC | 0:2 | ||||
| 42. Ronde | 15.04.2022 | Exeter City | - | Colchester United | 2:0 | ||||
| 45. Ronde | 30.04.2022 | Colchester United | - | Walsall FC | 2:2 |
Ierland