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| 17.02.2001 | 16:00 | Barnet FC | - | Darlington FC | 3:0 | |
| 16:00 | Blackpool FC | - | Lincoln City | 2:0 | ||
| 16:00 | Cardiff City | - | Scunthorpe United | 3:0 | ||
| 16:00 | Carlisle United | - | Plymouth Argyle | 1:1 | ||
| 16:00 | Cheltenham Town | - | Brighton & Hove Albion | 3:1 | ||
| 16:00 | Leyton Orient | - | Kidderminster Harriers | 0:0 | ||
| 16:00 | Macclesfield Town | - | Hartlepool United | 0:1 | ||
| 16:00 | Mansfield Town | - | Chesterfield FC | 0:1 | ||
| 16:00 | Shrewsbury Town | - | Hull City | 0:2 | ||
| 16:00 | Southend United | - | Halifax Town | 0:3 | ||
| 16:00 | Torquay United | - | Rochdale AFC | 1:0 | ||
| 16:00 | York City | - | Exeter City | 0:3 |
| » Stand | Thuis | uit | Heenronde | Returnronde |
| # | Team | GS. | W. | G. | V. | Dp. | Ds. | P. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Brighton & Hove Albion | 32 | 20 | 4 | 8 | 52:26 | 26 | 64 |
| 2 |
|
Chesterfield FC | 32 | 20 | 10 | 2 | 61:23 | 38 | 61 |
| 3 |
|
Cardiff City | 32 | 17 | 10 | 5 | 68:33 | 35 | 61 |
| 4 |
|
Rochdale AFC | 32 | 15 | 11 | 6 | 47:27 | 20 | 56 |
| 5 |
|
Hartlepool United | 32 | 16 | 8 | 8 | 52:39 | 13 | 56 |
| 6 |
|
Leyton Orient | 32 | 15 | 9 | 8 | 42:36 | 6 | 54 |
| 7 |
|
Blackpool FC | 32 | 16 | 4 | 12 | 50:40 | 10 | 52 |
| 8 |
|
Southend United | 32 | 12 | 13 | 7 | 38:34 | 4 | 49 |
| 9 |
|
Cheltenham Town | 32 | 13 | 7 | 12 | 39:36 | 3 | 46 |
| 10 |
|
Hull City | 32 | 11 | 13 | 8 | 30:27 | 3 | 46 |
| 11 |
|
Mansfield Town | 32 | 11 | 10 | 11 | 47:50 | -3 | 43 |
| 12 |
|
Scunthorpe United | 32 | 11 | 9 | 12 | 39:36 | 3 | 42 |
| 13 |
|
Plymouth Argyle | 32 | 10 | 10 | 12 | 39:43 | -4 | 40 |
| 14 |
|
Kidderminster Harriers | 32 | 9 | 12 | 11 | 31:40 | -9 | 39 |
| 15 |
|
Macclesfield Town | 32 | 9 | 11 | 12 | 38:44 | -6 | 38 |
| 16 |
|
Barnet FC | 32 | 10 | 7 | 15 | 51:55 | -4 | 37 |
| 17 |
|
Shrewsbury Town | 32 | 9 | 7 | 16 | 26:47 | -21 | 34 |
| 18 |
|
Darlington FC | 32 | 8 | 9 | 15 | 30:39 | -9 | 33 |
| 19 |
|
Torquay United | 32 | 8 | 9 | 15 | 36:58 | -22 | 33 |
| 20 |
|
Halifax Town | 32 | 8 | 8 | 16 | 36:48 | -12 | 32 |
| 21 |
|
Carlisle United | 32 | 7 | 9 | 16 | 33:51 | -18 | 30 |
| 22 |
|
York City | 32 | 8 | 6 | 18 | 25:49 | -24 | 30 |
| 23 |
|
Lincoln City | 32 | 6 | 11 | 15 | 36:47 | -11 | 29 |
| 24 |
|
Exeter City | 32 | 6 | 11 | 15 | 28:46 | -18 | 29 |
| Promotie | |
| Play-offs | |
| Degradatie |
