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| U | Olympique Lyon | - | Real Madrid | 1:0 (0:0) | ||
| R | Real Madrid | - | Olympique Lyon | 1:1 (1:0) | ||
| U: 16.02.2010 20:45 | R: 10.03.2010 20:45 | Winnaars: Olympique Lyon | ||||
| U | AC Milan | - | Manchester United | 2:3 (1:1) | ||
| R | Manchester United | - | AC Milan | 4:0 (1:0) | ||
| U: 16.02.2010 20:45 | R: 10.03.2010 20:45 | Winnaars: Manchester United | ||||
| U | Bayern München | - | ACF Fiorentina | 2:1 (1:0) | ||
| R | ACF Fiorentina | - | Bayern München | 3:2 (1:0) | ||
| U: 17.02.2010 20:45 | R: 09.03.2010 20:45 | Winnaars: Bayern München | ||||
| U | FC Porto | - | Arsenal FC | 2:1 (1:1) | ||
| R | Arsenal FC | - | FC Porto | 5:0 (2:0) | ||
| U: 17.02.2010 20:45 | R: 09.03.2010 20:45 | Winnaars: Arsenal FC | ||||
| U | VfB Stuttgart | - | FC Barcelona | 1:1 (1:0) | ||
| R | FC Barcelona | - | VfB Stuttgart | 4:0 (2:0) | ||
| U: 23.02.2010 20:45 | R: 17.03.2010 20:45 | Winnaars: FC Barcelona | ||||
| U | Olympiakos | - | Girondins Bordeaux | 0:1 (0:1) | ||
| R | Girondins Bordeaux | - | Olympiakos | 2:1 (1:0) | ||
| U: 23.02.2010 20:45 | R: 17.03.2010 20:45 | Winnaars: Girondins Bordeaux | ||||
| U | CSKA Moskou | - | Sevilla FC | 1:1 (0:1) | ||
| R | Sevilla FC | - | CSKA Moskou | 1:2 (1:1) | ||
| U: 24.02.2010 18:30 | R: 16.03.2010 20:45 | Winnaars: CSKA Moskou | ||||
| U | Inter | - | Chelsea FC | 2:1 (1:0) | ||
| R | Chelsea FC | - | Inter | 0:1 (0:0) | ||
| U: 24.02.2010 20:45 | R: 16.03.2010 20:45 | Winnaars: Inter | ||||
